In vitro antimicrobial susceptibility testing methods: agar dilution to 3D tissue-engineered models
نویسندگان
چکیده
منابع مشابه
Comparison of the Etest and agar dilution for in vitro antimicrobial susceptibility testing of Campylobacter.
The performance of the Etest and agar dilution for in vitro antimicrobial susceptibility of Campylobacter spp. was evaluated using a quality control strain Campylobactor jejuni ATCC 33560, and 81 C. jejuni and 54 Campylobacter coli isolates recovered from retail raw meats. Seven antimicrobial agents: chloramphenicol, ciprofloxacin, doxycycline, erythromycin, gentamicin, nalidixic acid and tetra...
متن کاملDetection with Agar Dilution Susceptibility Testing
A dye reduction method for determining a definitive endpoint with agar dilution susceptibility testing has been developed. Bacterial growth was determined by applying to the inoculum spot a dye solution containing 2-(p-iodophenyl)-3-(pnitrophenyl)-5-phenyltetrazolium chloride and phenazine methosulfate. Viable colonies reduced the tetrazolium salt to a visible red color within 3 to 5 min. The m...
متن کاملComparison of antimicrobial susceptibility testing of Campylobacter spp. by the agar dilution and the agar disk diffusion methods.
The correlation and the level of agreement between the standardized agar dilution and the agar disk diffusion methods for antimicrobial susceptibility testing of Campylobacter were investigated. A high-level agreement between the two methods was evident for aminoglycosides and fluoroquinolones, while a low-level agreement was observed for other antibiotics.
متن کاملIn vitro susceptibility of Bartonella species to 17 antimicrobial compounds: comparison of Etest and agar dilution.
OBJECTIVES In vitro susceptibility testing of 31 Bartonella spp. strains including 21 Bartonella henselae isolates was performed for 17 antimicrobial agents (telithromycin, four macrolides, five fluoroquinolones, five aminoglycosides, doxycycline and rifampicin). METHODS MICs were determined by agar dilution and Etest using chocolate agar containing 5% defibrinated sheep blood as assay medium...
متن کاملAssessment of Etest as an alternative to agar dilution for antimicrobial susceptibility testing of Neisseria gonorrhoeae.
We studied whether the Etest can be used as an alternative to agar dilution to determine antimicrobial susceptibilities of ceftriaxone, cefixime, and cefpodoxime in Neisseria gonorrhoeae surveillance. One hundred fifteen clinical and laboratory isolates of N. gonorrhoeae were tested following the Clinical Laboratory Improvement Amendments (CLIA)-approved CLSI standard agar dilution method and, ...
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ژورنال
عنوان ژورنال: European Journal of Clinical Microbiology & Infectious Diseases
سال: 2017
ISSN: 0934-9723,1435-4373
DOI: 10.1007/s10096-017-3089-2